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Study and development of multilayer needle-type enzyme-based glucose microsensors
R Sternberg1, M B Barrau, L Gangiotti
1Laboratoire de Bioélectrochimie et Analyse du Milieu, U.F.R. de Sciences et de Technologie, Université Paris-Val de Marne, Créteil, France.
Biosensors
|January 1, 1989
Summary
This study developed a novel glucose biosensor using glucose oxidase (GOD) immobilized on a cellulose acetate (CA) layer. The developed sensor shows good glucose sensitivity and partial discrimination against interfering substances for in vivo applications.
Area of Science:
- Biomedical Engineering
- Biosensor Technology
- Electrochemistry
Background:
- Accurate glucose monitoring is crucial for diabetes management.
- Development of stable and sensitive glucose biosensors remains an active research area.
- Covalent immobilization strategies enhance enzyme stability and sensor performance.
Purpose of the Study:
- To develop and characterize a novel needle-type glucose biosensor.
- To evaluate the performance of the biosensor in vitro and in vivo.
- To assess the sensor's ability to discriminate against interfering substances.
Main Methods:
- Covalent coupling of glucose oxidase (GOD) to a cellulose acetate (CA) layer using BSA and PBQ linkages.
- Fabrication of a multilayer microsensor with a polyurethane (PU) outer layer on a platinum electrode.
- In vitro characterization of GOD loading, glucose response, and linear range.
- In vivo implantation in anesthetized rats for subcutaneous glucose monitoring.
- Evaluation of anion discrimination against hydrogen peroxide.
Main Results:
- Successfully immobilized GOD onto the CA layer, achieving a GOD load of 1.6-3.0 µg mm⁻².
- Demonstrated significant glucose response (1-3 µA M⁻¹ mm⁻²) with a linear range of 10-30 mM.
- Observed partial discrimination of small anions like ascorbate from neutral molecules like hydrogen peroxide.
- In vivo sensor responses correlated with blood glucose levels but required a two-point calibration due to differing sensitivity coefficients compared to in vitro measurements.
Conclusions:
- The developed needle-type glucose biosensor exhibits robust performance and stability.
- The multilayer structure provides partial selectivity against common interferents.
- In vivo application necessitates specific calibration strategies to account for physiological differences.
- This biosensor holds potential for continuous glucose monitoring in diabetic patients.