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Direct PCR of Intact Bacteria (Colony PCR)
Michael E Woodman1,2, Christina R Savage1, William K Arnold1
1Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, Lexington, Kentucky.
Current Protocols in Microbiology
|August 13, 2016
Abstract:
This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
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