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Related Concept Videos

Transcription Elongation Factors02:35

Transcription Elongation Factors

14.4K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.4K
Transcription Elongation Factors02:35

Transcription Elongation Factors

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Transcription Initiation01:47

Transcription Initiation

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Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
21.9K
Bacterial Transcription01:53

Bacterial Transcription

38.4K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
38.4K
Transcription01:17

Transcription

35.0K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
35.0K
Transcription01:10

Transcription

158.3K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.3K

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Related Experiment Video

Updated: Mar 16, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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SnapShot: Transcription Elongation.

Christine E Cucinotta1, Karen M Arndt1

  • 1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Cell
|August 13, 2016
PubMed
Summary

Transcription elongation, a key gene expression step, is regulated by proteins and mechanisms like RNA Polymerase II pausing and histone modifications.

Area of Science:

  • Molecular Biology
  • Gene Regulation

Background:

  • Transcription elongation is a critical control point in gene expression.
  • This process involves complex regulatory mechanisms.
  • Key players include RNA Polymerase II and histone modifications.

Purpose of the Study:

  • To elucidate the regulatory mechanisms governing transcription elongation.
  • To understand the roles of specific proteins and modifications in this process.

Main Methods:

  • Investigated RNA Polymerase II pausing dynamics.
  • Analyzed cotranscriptional histone modification patterns.
  • Utilized biochemical and genetic approaches.

Main Results:

  • Identified key protein factors influencing elongation rates.

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In Vitro Transcription Assays and Their Application in Drug Discovery
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In Vitro Transcription Assays and Their Application in Drug Discovery

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Measuring the Kinetics of mRNA Transcription in Single Living Cells
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Measuring the Kinetics of mRNA Transcription in Single Living Cells

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Last Updated: Mar 16, 2026

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In Vitro Transcription Assays and Their Application in Drug Discovery
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  • Characterized the interplay between histone modifications and polymerase activity.
  • Demonstrated the significance of pausing in gene regulation.

    • Conclusions:

    • Transcription elongation is a highly regulated process.
    • Protein factors and histone modifications are crucial for controlling gene expression.
    • Understanding these mechanisms provides insights into gene regulation.