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Updated: Mar 16, 2026
Quantification of γH2AX Foci in Response to Ionising Radiation
Published on: April 6, 2010
Determination of Histone H2AX Phosphorylation in DT40 Cells
Kana Nishihara1, Sampada A Shahane1, Menghang Xia2
1National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
Abstract:
Visualization of DNA damage response protein recruitment to DNA damage sites enables measurement of the DNA damage. DNA double-strand breaks (DSBs) and blocked replication forks induce the phosphorylation of H2AX at serine 139 (γH2AX), and accumulate γH2AX which can then be detected as foci. The detection of γH2AX foci by immunostaining with antibodies that recognize γH2AX is an indicator of DSBs presence. This chapter describes the measurement of γH2AX immunostaining using a high-content imaging platform in chicken DT40 B-lymphocyte cell lines.
