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Updated: Mar 16, 2026
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
Published on: May 13, 2019
High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells
Sampada A Shahane1, Kana Nishihara1, Menghang Xia2
1National Center for Advancing Translational Sciences, National Institutes of Health, Building C, MSC: 3375, 9800 Medical Center Drive, Bethesda, MD, 20892, USA.
None:
Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.
