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Adherence of Staphylococcus epidermidis to intraocular lenses
P G Griffiths1, T S Elliot, L McTaggart
1Department of Ophthalmology, Newcastle General Hospital, Newcastle upon Tyne.
Abstract:
We have demonstrated, with an in vitro model, that Staphylococcus epidermidis is able to colonise intraocular lenses. Adherent organisms were quantitated by light microscopy, scanning electron microscopy, and viable counting. Bacterial adherence was associated with production of a polysaccharide glycocalyx. Organisms which were attached to the lenses were resistant to apparently bactericidal concentrations of antibiotics, as determined by conventional testing. We speculate on the role of colonisation in the pathogenesis of endophthalmitis.
Insights
Staphylococcus epidermidis can colonize intraocular lenses, forming a protective glycocalyx. This bacterial colonization makes them resistant to antibiotics, potentially contributing to endophthalmitis.
Area of Science:
- Ophthalmology
- Microbiology
- Biomaterials Science
Background:
- Intraocular lenses (IOLs) are susceptible to microbial colonization.
- Staphylococcus epidermidis is a common cause of ocular infections.
Purpose of the Study:
- To investigate the ability of Staphylococcus epidermidis to colonize IOLs in vitro.
- To assess the impact of colonization on antibiotic resistance.
Main Methods:
- In vitro model using intraocular lenses.
- Quantification of bacterial adherence via light microscopy, scanning electron microscopy, and viable counts.
- Assessment of antibiotic resistance of adherent bacteria.
Main Results:
- Staphylococcus epidermidis successfully colonized intraocular lenses.
- Bacterial adherence was linked to the production of a polysaccharide glycocalyx.
- Colonized bacteria exhibited resistance to bactericidal antibiotic concentrations.
Conclusions:
- Staphylococcus epidermidis can form biofilms on IOLs.
- The glycocalyx contributes to antibiotic resistance in this model.
- IOL colonization by S. epidermidis may play a role in the development of endophthalmitis.