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Updated: Mar 16, 2026
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Measurement in vitro of the esterification of yeast sterols
1Department of Microbiology, Oregon State University, 97331, Corvallis, Oregon.
Abstract:
A highly reproducible and rapid procedure is described for isolating free sterols and sterol esters from cell-free extracts of yeast. Using this technique, the sterol esterifying enzyme has been studied. Sterol ester hydrolase has also been demonstrated. In agreement with whole cell experiments, the enzyme activity for sterol ester synthesis has been found to increase on entrance of the culture into the stationary phase of growth.
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