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Updated: Mar 16, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Effect of catecholamines and β-blockers on linoleic acid desaturation activity
I N de Gómez Dumm1, M J de Alaniz1, R R Brenner1
1Instituto de Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, calle 60 y 120, La Plata, Argentina.
Abstract:
The effect of catecholamines and adrenergic blocking agents on the oxidative desaturation of linoleic acid in rat liver microsomes was studied. Epinephrine (1 mg/kg/body weight) produced a significant decrease on the conversion of [1-(14)C]linoleic acid to γ-linolenic acid. The effect of epinephrine was blocked by single injections of the β blockers propranolol (10 mg/kg body weight) or dichloroisoproterenol 30 min before the hormone treatment. Isoproterenol (100 μg/kg body weight) produced a significant decrease on the activity of the linoleyl-CoA desaturase. The effect of the catecholamines was postulated to be mediated through β receptors by an enhancement of the intracellular levels of cyclic AMP.
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