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Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
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Calreticulin in the heart.

Marek Michalak1, Lei Guo1, Murray Robertson2

  • 1Canadian Institutes of Health Research, Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.

Molecular and Cellular Biochemistry
|August 14, 2016
PubMed
Summary

Calreticulin, an endoplasmic reticulum protein, is crucial for heart development. Its absence is lethal, while its over-expression causes severe cardiac dysfunction and sudden death.

Keywords:
calcium binding proteinscardiac arrhythmiacardiac developmentchaperoneendoplasmic reticulum

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Cardiology

Background:

  • Calreticulin is a Ca(2+) binding chaperone in the endoplasmic reticulum.
  • It is essential for the calreticulin/calnexin cycle and ER quality control.
  • Calreticulin plays a vital role in cardiac development and function.

Purpose of the Study:

  • To investigate the role of calreticulin in cardiac development and postnatal pathologies.
  • To understand the consequences of calreticulin deficiency and over-expression in the heart.

Main Methods:

  • The study likely involved analyzing cardiac development in the presence and absence of calreticulin.
  • Ultrastructural analysis was used to examine cardiac tissue.
  • The study may have involved genetic manipulation to alter calreticulin levels.

Main Results:

  • Calreticulin deficiency is lethal, causing impaired cardiac development.
  • Over-expression of calreticulin leads to bradycardia, heart block, and sudden death.
  • Absence of calreticulin may result in defects in contractile and conductive systems, and metabolic abnormalities.

Conclusions:

  • Calreticulin is indispensable for normal cardiac development.
  • Both deficiency and over-expression of calreticulin result in severe cardiac pathologies.
  • The endoplasmic reticulum and calreticulin are critical for maintaining cardiac health throughout development and life.