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Factors influencing cellulase induction inFusarium sp
1Department of Microbiology, M. S. University of Baroda, 390 001, Baroda, India.
Current Microbiology
|August 14, 2016
Summary
Fusarium sp. showed reduced lag time for cellulase production when pre-grown on lactose versus glucose. Insoluble cellulose maximized enzyme output, with glucose addition repressing cellulase activity.
Area of Science:
- Microbiology
- Enzymology
- Biochemistry
Background:
- Cellulase enzymes are crucial for breaking down cellulose.
- Understanding cellulase induction in fungi like Fusarium sp. is important for biotechnological applications.
- Catabolite repression is a known regulatory mechanism in microbial enzyme production.
Purpose of the Study:
- To investigate the induction of cellulase in Fusarium sp.
- To determine the optimal conditions for cellulase production.
- To explore the role of carbon sources and catabolite repression in regulating cellulase synthesis.
Main Methods:
- Culturing Fusarium sp. with different carbon sources (lactose, glucose, insoluble cellulose, carboxymethyl cellulose).
- Measuring cellulase activity in the culture filtrate.
- Analyzing the effect of supplementary carbon sources and step-feeding on enzyme production and cell growth.
Main Results:
- Lactose-pregrown cells exhibited a reduced lag period for cellulase induction compared to glucose-pregrown cells.
- Insoluble cellulose (Sigmacell) resulted in maximal cellulase production.
- Supplementation with cellobiose or glucose to cellulose-grown cultures increased biomass but decreased cellulase production.
- Cellulase activity was detected only after glucose depletion in Sigmacell-supplemented media, indicating catabolite repression.
Conclusions:
- Cellulase production in Fusarium sp. is influenced by the carbon source used for pre-growth and induction.
- Insoluble cellulose is a potent inducer of cellulase in this organism.
- Cellulase synthesis in Fusarium sp. appears to be subject to catabolite repression by glucose.
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