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Osteogenic cell response to calcium aluminate-based cement
L M S Castro-Raucci1,2, L N Teixeira1,3, I R Oliveira4
1School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
International Endodontic Journal
|August 14, 2016
Summary
Calcium aluminate-based cement (CAC+) promotes osteogenic cell phenotype development in vitro, showing potential as an alternative to Mineral Trioxide Aggregate (MTA) in endodontic treatments.
Area of Science:
- Biomaterials Science
- Regenerative Dentistry
- Cell Biology
Background:
- Mineral Trioxide Aggregate (MTA) is a common endodontic material.
- There is a need for alternative biomaterials in endodontic procedures.
- Calcium aluminate-based cement (CAC+) is a novel material for evaluation.
Purpose of the Study:
- To assess the in vitro impact of CAC+ on osteogenic phenotype development.
- To compare the effects of CAC+ with MTA on osteogenic cells.
Main Methods:
- Rat calvaria-derived cells were cultured and exposed to CAC+ or MTA samples.
- Cell viability, proliferation, and differentiation markers were analyzed over 14 days.
- Statistical analysis included Kruskal-Wallis and Student-Newman-Keuls tests.
Main Results:
- Both CAC+ and MTA inhibited cell growth in close proximity.
- At a distance, CAC+ significantly enhanced cell viability, alkaline phosphatase (ALP) activity, and expression of key osteogenic markers compared to MTA.
- Matrix mineralization was not significantly affected by CAC+ exposure.
Conclusions:
- CAC+ effectively supports osteogenic cell phenotype acquisition in vitro.
- CAC+ shows promise as a viable alternative to MTA for endodontic applications.
- Further in vivo studies are required to confirm bone repair acceleration.
Keywords:
calcium aluminate cementcell differentiationgene expressionmineral trioxide aggregateosteoblastsroot canal filling materialsMore Related Videos
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