Assessing cadmium and vanadium accumulation using diffusive gradient in thin-films (DGT) and phytoplankton in the...

Diffusive gradient in thin films (DGT) and phytoplankton communities were evaluated for the measurement of Cd and V at environmentally relevant concentrations in laboratory settings and in the Churchill River estuary (Manitoba, Canada) during an annual spring melt. Despite rapid changes in hydrology and water quality, DGT samplers and intracellular Cd and V concentrations were positively correlated (0.79 < r(2) < 0.99), suggesting comparable accumulation trends between both DGT-labile and intracellular monitoring techniques. The largest accumulated concentrations of both Cd and V by DGT and phytoplankton accumulation methods were found later into the river discharge period. In controlled settings, accumulated Cd and V concentrations by the diatom Attheya septentrionalis displayed a strong correlation with metals accumulated by DGTs (r(2) > 0.99). Principal component analysis (PCA) reinforced similarities between both metal monitoring techniques and assessed how changing environmental variables during the river discharge period influenced each monitoring technique. Cd accumulation was influenced by DOC concentrations and protein-like DOM whereas ionic strength (i.e. conductivity) and humic-like DOM influenced V accumulation. The present findings suggest that (1) DGT is a versatile tool for monitoring bioaccumulation of Cd and V in highly dynamic environmental systems and (2) DOC concentration, DOM composition, conductivity, pH, and river discharge influence the bioavailability of Cd and V in estuarine and riverine waters.