[Immunohistochemical detection of triiodothyronine in thyroid tumors using T3-01 monoclonal antibody]

Cells of the spleen of BALB/c mice immunized with a conjugate of triiodothyronine and bovine immunoglobulin were fused with cells of the murine myeloma line F O. Selection and cloning were used to look up the T3-01 hybridoma producing a monoclonal antibody of the IgG1 subclass which responded in a radioimmunoassay specifically to triiodothyronine. The T3-01 monoclonal antibody was used for the immunohistochemical demonstration of triiodothyronine on 19 tumour derivatives.