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Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
Published on: July 26, 2019
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Siglec-F is a novel intestinal M cell marker
Nadezhda Gicheva1, Matthew S Macauley2, Britni M Arlian3
1Food and Health Institute Strategic Program, Institute of Food Research, Norwich, UK.
Biochemical and Biophysical Research Communications
|August 16, 2016
Summary
Researchers identified Sialic acid-binding immunoglobulin-like lectin F (Siglec-F) as a target on intestinal microfold (M) cells. This discovery offers a novel strategy for oral vaccine delivery by enabling targeted M cell delivery.
Area of Science:
- Immunology
- Gastroenterology
- Vaccinology
Background:
- Intestinal microfold (M) cells in Peyer's patches (PPs) are crucial for shuttling antigens to initiate immune responses.
- M cells are a key target for developing next-generation oral vaccines.
- Identifying M cell-specific receptors is vital for targeted antigen delivery.
Purpose of the Study:
- To identify novel M cell-specific receptors for targeted oral vaccine delivery.
- To investigate the expression of Sialic acid-binding immunoglobulin-like lectin F (Siglec-F) on intestinal M cells.
Main Methods:
- Generation of a monoclonal antibody against Siglec-F.
- Immunohistochemical analysis of mouse Peyer's patch tissue sections.
- Intestinal administration of anti-Siglec-F antibody in mice.
Main Results:
- Siglec-F is expressed on the surface of mouse intestinal M cells, facing the lumen.
- The anti-Siglec-F antibody successfully bound to M cells after injection into the mouse small intestine.
Conclusions:
- Siglec-F is a potential M cell-specific marker.
- Targeting Siglec-F presents a promising approach for M cell-directed oral vaccine delivery.
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