Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

7.0K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
7.0K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

6.1K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
6.1K
Protein Transport into the Inner Mitochondrial Membrane01:34

Protein Transport into the Inner Mitochondrial Membrane

5.1K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
5.1K
Insertion of Single-pass Transmembrane Proteins in the RER01:26

Insertion of Single-pass Transmembrane Proteins in the RER

18.7K
Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
18.7K
Insertion of Multi-pass Transmembrane Proteins in the RER01:29

Insertion of Multi-pass Transmembrane Proteins in the RER

19.3K
The rough ER membrane synthesizes, assembles, and embeds transmembrane proteins in diverse topologies. These proteins function as transporters or channels and can remain in the ER membrane or are sent to the Golgi complex, lysosome, and cell membrane.
The multipass transmembrane proteins are the type IV integral membrane proteins with multiple topogenic sequences determining their spatial arrangement in the ER membrane. Nearly all multipass proteins lack a cleavable signal sequence and use...
19.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two types of microplastics (polystyrene-HBCD and car tire abrasion) affect oxidative stress-related biomarkers in earthworm Eisenia andrei in a time-dependent manner.

Environment international·2022
Same author

Determination of tire wear markers in soil samples and their distribution in a roadside soil.

Chemosphere·2022
Same author

Beneficial effects of dynamic groundwater flow and redox conditions on Natural Attenuation of mono-, poly-, and NSO-heterocyclic hydrocarbons.

Journal of contaminant hydrology·2021
Same author

A multi-centre, randomized, controlled trial on coaching and telemonitoring in patients with cystic fibrosis: conneCT CF.

BMC pulmonary medicine·2021
Same author

Quality of life in patients with limited (1-3) brain metastases undergoing stereotactic or whole brain radiotherapy : A prospective study of the DEGRO QoL working group.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2019
Same author

Ecotoxicity of Nitrogen, Sulfur, or Oxygen Heterocycles and Short-Chained Alkyl Phenols Commonly Detected in Contaminated Groundwater.

Environmental toxicology and chemistry·2019
Same journal

Defining and characterizing the relevant state variables of the mammalian gut ecosystem.

Cell reports·2026
Same journal

Distinct compositional changes but shared quantitative microbiome and anti-inflammatory modulations by diet.

Cell reports·2026
Same journal

HPD is a copper-binding protein that interacts with DLAT to promote colorectal cancer cuproptosis under copper stress.

Cell reports·2026
Same journal

Rotational trophoblast organoids reveal biomechanical regulation of trophoblast differentiation.

Cell reports·2026
Same journal

Dysregulated calcium signaling underlies hyposalivation and microbial dysbiosis in Down syndrome.

Cell reports·2026
Same journal

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer.

Cell reports·2026
See all related articles

Related Experiment Video

Updated: Mar 16, 2026

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
05:28

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Published on: September 10, 2020

2.8K

A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency.

Stephen S Marshall1, Michiel J M Niesen1, Axel Müller1

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.

Cell Reports
|August 16, 2016
PubMed
Summary
This summary is machine-generated.

Integral membrane proteins (IMPs) expression is challenging. Protein sequence impacts IMP integration efficiency, directly correlating with expression levels, enabling rational design for improved protein production.

More Related Videos

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

15.7K
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
08:38

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Published on: August 14, 2011

22.6K

Related Experiment Videos

Last Updated: Mar 16, 2026

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
05:28

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Published on: September 10, 2020

2.8K
Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

15.7K
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
08:38

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Published on: August 14, 2011

22.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Integral membrane proteins (IMPs) are crucial for cellular functions but difficult to express.
  • Understanding factors influencing IMP expression is vital for mechanistic studies.

Purpose of the Study:

  • To investigate the relationship between protein sequence and expression levels of IMPs.
  • To determine if simulated integration efficiency can predict experimental IMP expression.

Main Methods:

  • Coarse-grained modeling to simulate protein integration efficiency.
  • Experimental assays in Escherichia coli and Mycobacterium smegmatis to measure IMP expression levels.
  • Sequence analysis of IMP homologs, specifically TatC.

Main Results:

  • Small sequence variations in IMPs significantly affect observed expression levels.
  • Simulated integration efficiency strongly correlates with experimentally determined expression levels.
  • Mutations improving simulated integration also enhance experimental expression.

Conclusions:

  • Protein integration efficiency is a key determinant of successful IMP expression.
  • Rational design based on sequence and integration efficiency can control IMP expression.
  • Findings are generalizable across different expression systems.