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Differences in Intracellular Fate of Two Spotted Fever Group Rickettsia in Macrophage-Like Cells
Pedro Curto1, Isaura Simões2, Sean P Riley3
1PhD Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology, University of CoimbraCoimbra, Portugal; Institute for Interdisciplinary Research, University of CoimbraCoimbra, Portugal; Center for Neuroscience and Cell BiologyCoimbra, Portugal; Vector Borne Disease Laboratories, Department of Pathobiological Sciences, LSU School of Veterinary MedicineBaton Rouge, LA, USA.
Abstract:
Spotted fever group (SFG) rickettsiae are recognized as important agents of human tick-borne diseases worldwide, such as Mediterranean spotted fever (Rickettsia conorii) and Rocky Mountain spotted fever (Rickettsia rickettsii). Recent studies in several animal models have provided evidence of non-endothelial parasitism by pathogenic SFG Rickettsia species, suggesting that the interaction of rickettsiae with cells other than the endothelium may play an important role in pathogenesis of rickettsial diseases. These studies raise the hypothesis that the role of macrophages in rickettsial pathogenesis may have been underappreciated. Herein, we evaluated the ability of two SFG rickettsial species, R. conorii (a recognized human pathogen) and Rickettsia montanensis (a non-virulent member of SFG) to proliferate in THP-1 macrophage-like cells, or within non-phagocytic cell lines. Our results demonstrate that R. conorii was able to survive and proliferate in both phagocytic and epithelial cells in vitro. In contrast, R. montanensis was able to grow in non-phagocytic cells, but was drastically compromised in the ability to proliferate within both undifferentiated and PMA-differentiated THP-1 cells. Interestingly, association assays revealed that R. montanensis was defective in binding to THP-1-derived macrophages; however, the invasion of the bacteria that are able to adhere did not appear to be affected. We have also demonstrated that R. montanensis which entered into THP-1-derived macrophages were rapidly destroyed and partially co-localized with LAMP-2 and cathepsin D, two markers of lysosomal compartments. In contrast, R. conorii was present as intact bacteria and free in the cytoplasm in both cell types. These findings suggest that a phenotypic difference between a non-pathogenic and a pathogenic SFG member lies in their respective ability to proliferate in macrophage-like cells, and may provide an explanation as to why certain SFG rickettsial species are not associated with disease in mammals.
Insights
Spotted fever group rickettsiae pathogenesis differs based on macrophage interaction. Pathogenic Rickettsia conorii proliferates in macrophages, unlike non-pathogenic Rickettsia montanensis, explaining disease association differences.
Area of Science:
- Microbiology
- Pathogenesis
- Cell Biology
Background:
- Spotted fever group (SFG) rickettsiae cause tick-borne diseases globally.
- Recent evidence suggests non-endothelial cell interactions are key to SFG rickettsiae pathogenesis.
- The role of macrophages in rickettsial disease pathogenesis may be underestimated.
Purpose of the Study:
- To investigate the role of macrophages in SFG rickettsiae pathogenesis.
- To compare the ability of pathogenic Rickettsia conorii and non-virulent Rickettsia montanensis to proliferate in macrophage-like cells and non-phagocytic cells.
Main Methods:
- In vitro proliferation assays using THP-1 macrophage-like cells and non-phagocytic cell lines.
- Bacterial association and invasion assays with THP-1 derived macrophages.
- Co-localization studies using LAMP-2 and cathepsin D markers.
Main Results:
- Rickettsia conorii survived and proliferated in both phagocytic and epithelial cells.
- Rickettsia montanensis showed limited proliferation in THP-1 cells, with defective binding and rapid destruction within lysosomes.
- Rickettsia conorii survived freely in the cytoplasm of both cell types.
Conclusions:
- A key difference between pathogenic and non-pathogenic SFG rickettsiae lies in their ability to proliferate within macrophages.
- Macrophage interaction and survival mechanisms may explain the varying disease association of different SFG rickettsial species.
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