Related Experiment Video
Updated: Mar 16, 2026
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Published on: November 14, 2016
MiniSOG-mediated Photoablation in Caenorhabdtis elegans
1Division of Biological Sciences, Neurobiology Section, University of California, San Diego, CA, USA.
Abstract:
This protocol describes a method for light-inducible cell ablation in live worms. miniSOG (mini Singlet Oxygen Generator) generates singlet oxygen upon blue light illumination (Shu et al., 2011). Mitochondrially membrane targeted miniSOG (the first 55 a. a. of C. e. tomm-20 fused at the N'-terminus of miniSOG, termed as mito-miniSOG in the following) is transgenically expressed in specific cells/tissues (Qi et al., 2012). Groups of transgenic animals are illuminated under open field fluorescence light on a compound microscope or LED light setup for photo-ablation.
