Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.9K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.9K
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

82
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
82
Experimental RNAi02:15

Experimental RNAi

8.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of sodium thiosulfate in prevention of aki in oncologic patient that underwent hipec (Hyperthermic Intraperitoneal Chemotherapy) with Cisplatin: a Case Report.

La Clinica terapeutica·2026
Same author

Non operative management of postpartum Diastasis Recti: a systematic review and metanalysis of randomized controlled trials.

Hernia : the journal of hernias and abdominal wall surgery·2026
Same author

Chitosan/halloysite clay mixture for preservation of waterlogged archaeological woods.

International journal of biological macromolecules·2025
Same author

Intraoperative nerve monitoring in thyroid and parathyroid surgery: a decade of Italian practice.

Updates in surgery·2025
Same author

Complex abdomen: a scoping review.

Hernia : the journal of hernias and abdominal wall surgery·2025
Same author

Observed-to-expected lung-area-to-head-circumference ratio on ultrasound examination vs total fetal lung volume on magnetic resonance imaging in prediction of survival in fetuses with left-sided diaphragmatic hernia.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2024

Related Experiment Video

Updated: Mar 16, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K

Smart Inulin-Based Polycationic Nanodevices for siRNA Delivery.

G Cavallaro1, C Sardo, C Scialabba

  • 1Lab of Biocompatible Polymers, Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, via Archirafi 32, Palermo 90123, Italy.

Current Drug Delivery
|August 17, 2016
PubMed
Summary

Inulin-based nanodevices effectively deliver short interfering RNA (siRNA) for gene silencing therapies. These biocompatible systems show promise for treating diseases like cancer by targeting gene expression.

Keywords:
BiocompatibilitysiRNA complexing abilitysilencing ability

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

8.3K

Related Experiment Videos

Last Updated: Mar 16, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.0K
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

8.3K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Gene Therapy

Background:

  • Short interfering RNA (siRNA) offers therapeutic potential by silencing disease-related genes.
  • Developing effective delivery systems for siRNA is crucial for its clinical application.
  • Inulin, a biocompatible polysaccharide, is explored as a scaffold for nucleic acid-based drug delivery systems.

Purpose of the Study:

  • To design and evaluate inulin-based nanodevices for siRNA delivery.
  • To compare different inulin derivatives functionalized with oligoamines for their efficacy.
  • To assess the chemical structure, biocompatibility, and gene silencing capabilities of these nanodevices.

Main Methods:

  • Synthesis of inulin derivatives functionalized with oligoamines (e.g., EDA, DETA, SPM).
  • Formation of polyplexes and polymeric coatings for nanocarriers.
  • Characterization of nanodevices for structure, siRNA complexation, and biocompatibility.
  • Evaluation of gene silencing efficacy in relevant models.

Main Results:

  • Inulin-based nanodevices were successfully synthesized and characterized.
  • Functionalized inulin demonstrated effective complexation with siRNA.
  • The nanodevices exhibited good biocompatibility and potent gene silencing activity.
  • Comparison revealed differences in performance based on chemical design and structure.

Conclusions:

  • Inulin-based nanodevices represent a promising platform for siRNA delivery.
  • Tailoring the chemical design of inulin derivatives enhances their therapeutic potential.
  • These nanocarriers offer a biocompatible and effective strategy for gene silencing therapies, particularly in cancer treatment.