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Updated: Mar 16, 2026
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
Correlation of Checkerboard Synergy Testing with Time-Kill Analysis and Clinical Outcomes of Extensively
Derek N Bremmer1, Karri A Bauer1, Stephanie M Pouch2
1Department of Pharmacy, The Ohio State University Wexner Medical Center, Columbus, Ohio, USA.
Abstract:
We tested 76 extensively drug-resistant (XDR) Acinetobacter baumannii isolates by the checkerboard method using only wells containing serum-achievable concentrations (SACs) of drugs. Checkerboard results were correlated by time-kill assay and clinical outcomes. Minocycline-colistin was the best combination in vitro, as it inhibited growth in one or more SAC wells in all isolates. Patients who received a combination that inhibited growth in one or more SAC wells demonstrated better microbiological clearance than those who did not (88% versus 30%; P = 0.025). The checkerboard platform may have clinical utility for XDR A. baumannii infections.
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