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Simple, Affordable, and Modular Patterning of Cells using DNA

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Parallelized cytoindentation using convex micropatterned surfaces.

Bojing Jia^1,2, Tse-Luen Wee^2,3,4, Colton G Boudreau³

¹Department of Physics.

|August 17, 2016

Summary

This summary is machine-generated.

A new high-throughput cytoindentor uses convex lens-induced confinement (CLiC) to precisely compress live cells. This advanced tool enables efficient, non-damaging mechanical studies of cellular properties, advancing mechanobiology research.

Keywords:

cell loading confocal microscopy convex lens-induced confinement cytoindentation microfabrication

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Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Mechanical forces significantly influence cellular behavior and function.
Existing methods for probing cellular mechanics, like atomic force microscopy (AFM), are often low-throughput and limited to single cells.

Purpose of the Study:

To develop a high-throughput, parallelized cytoindentor for local compression of live cells.
To enable precise mechanical manipulation of subcellular regions without causing cell damage.

Main Methods:

Utilized convex lens-induced confinement (CLiC) with micropatterned poly(dimethylsiloxane) (PDMS) films to create arrays of posts.
Applied these posts for local indentation of single cells and cell populations with submicron precision.
Functionalized micropost arrays with glycoproteins like fibronectin for combined pulling and compression.

Main Results:

CLiC indentation induced local cell deformation without lysis.
Functionalized posts allowed customized confinement geometries for cell manipulation.
Measurements confirmed no significant cell damage or stress in Chinese hamster ovary (CHO-K1) cells.

Conclusions:

The CLiC cytoindentor is a novel, high-throughput tool for studying cellular mechanobiology and mechanochemistry.
This technique streamlines investigations by overcoming the single-cell limitations of traditional methods.
Opens new avenues for visualizing and understanding the mechanical properties of living cells.