Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prediction of the effect of biochar on soil CEC improvement based on machine learning.

Scientific reports·2026

Same author

Effects of crutch-assisted walking on pain, gait, and hip function in early and middle-stage osteonecrosis: a randomized trial.

Journal of orthopaedic translation·2026

Same author

Case Report: Report of 2 cases of periprosthetic joint infection caused by Brucella after joint replacement and literature review.

Frontiers in surgery·2026

Same author

ZmMS1 coordinates ROS homeostasis, lipid allocation, and male fertility for maize breeding applications.

Journal of integrative plant biology·2026

Same author

Emodin inhibits the formation of gallstones by affecting the intestinal flora and expression of hepatic HIF1α.

International immunopharmacology·2026

Same author

Theoretical evidence for enhanced piezoelectric properties of BY- or NaV-codoped wurtzite AlN.

Physical chemistry chemical physics : PCCP·2026

Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026

Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026

Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

Highly efficient sample stacking by enhanced field amplification on a simple paper device.

Biao Ma¹, Yi-Zhen Song, Ji-Cheng Niu

¹Research Center for Analytical Sciences, Chemistry Department, College of Sciences, Northeastern University, Shenyang 110819, PR China. zywu@mail.neu.edu.cn.

|August 17, 2016

Summary

This summary is machine-generated.

We developed a new paper-based electrokinetic stacking (ES) method for sample preconcentration. This technique significantly improves signal detection for analytes like DNA, enabling sensitive point-of-care testing.

More Related Videos

Picoinjection of Microfluidic Drops Without Metal Electrodes

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Related Experiment Videos

Last Updated: Mar 16, 2026

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

Picoinjection of Microfluidic Drops Without Metal Electrodes

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Area of Science:

Analytical Chemistry
Biotechnology
Materials Science

Background:

Paper-based analytical devices (PADs) offer low-cost solutions for diagnostics.
Effective sample preconcentration is crucial for enhancing sensitivity in PADs.
Existing preconcentration methods can be complex or time-consuming.

Purpose of the Study:

To introduce a novel, simple, and efficient electrokinetic stacking (ES) method for sample preconcentration.
To demonstrate the application of this ES method on paper-based devices.
To achieve significant signal enhancement for sensitive detection.

Main Methods:

Developed an electrokinetic stacking (ES) method utilizing field amplification on a paper substrate.
Applied voltage to induce stacking of charged probe ions at the interface of solutions with different conductivities.
Investigated the focusing effect balancing electromigration and electroosmotic flow for enhanced stacking.
Tested the method with fluorescein and double-stranded DNA.

Main Results:

Achieved a three-orders-of-magnitude signal improvement for fluorescein and DNA within 300 seconds.
Demonstrated rapid formation and stabilization of the stacking band.
Successfully applied the ES method to various low-cost substrates like cloth and thread.
Confirmed the focusing effect contributing to enhanced stacking efficiency.

Conclusions:

The novel paper-based ES method provides a simple and highly efficient approach for sample preconcentration.
This technique offers significant signal enhancement, paving the way for sensitive detection in PADs.
The method's versatility and low cost make it suitable for point-of-care testing (POCT) applications.