Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.9K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.9K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.6K
4.6K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
2.9K
Protein Folding01:25

Protein Folding

12.1K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
12.1K
Protein Folding01:22

Protein Folding

129.8K
Overview
129.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matrix structure and microenvironment dynamics correlate with chemotherapy response in ovarian cancer.

iScience·2026
Same author

3D pentaculture model unveils malignant cell-driven macrophage polarization in high-grade serous ovarian cancer.

Nature communications·2026
Same author

Variable Adherence to Australian Clinical Care Standard: An Audit of Patients With Acute Low Back Pain Attending a Tertiary Emergency Department.

Emergency medicine Australasia : EMA·2025
Same author

Navitoclax, a Bcl-2/xL Inhibitor, and YM155, a Survivin Inhibitor, in Combination with Carboplatin, Effectively Inhibit Ovarian Cancer Tumor Growth.

Molecular cancer therapeutics·2025
Same author

Human 3D Ovarian Cancer Models Reveal Malignant Cell-Intrinsic and -Extrinsic Factors That Influence CAR T-cell Activity.

Cancer research·2024
Same author

Collagen-I influences the post-translational regulation, binding partners and role of Annexin A2 in breast cancer progression.

Frontiers in oncology·2023

Related Experiment Video

Updated: Mar 16, 2026

Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

979

Studying protein-protein interactions: progress, pitfalls and solutions.

Sheri Hayes1, Beatrice Malacrida1, Maeve Kiely2

  • 1Graduate Entry Medical School and Health Research Institute, University of Limerick, Limerick, Ireland.

Biochemical Society Transactions
|August 17, 2016
PubMed
Summary
This summary is machine-generated.

This review guides researchers on selecting methods to study protein-protein interactions (PPIs), crucial for understanding cellular networks and developing disease therapies. It details various techniques for identifying and validating these transient biological interactions.

Keywords:
cell biologyprotein–protein interactionsproteomicssignalling pathways

More Related Videos

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.7K

Related Experiment Videos

Last Updated: Mar 16, 2026

Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

979
Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.8K
Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.7K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Signalling proteins orchestrate biological processes through complex interactions.
  • Understanding protein-protein interactions (PPIs) is vital for elucidating cellular networks and disease mechanisms.
  • Studying transient PPIs presents significant experimental challenges.

Purpose of the Study:

  • To provide researchers with a starting point for planning protein-protein interaction studies.
  • To offer an overview of available methods for identifying and validating PPIs.
  • To discuss the advantages and disadvantages of various PPI detection techniques.

Main Methods:

  • In silico analysis for predicting potential interactions.
  • Large-scale screening tools for high-throughput PPI identification.
  • Experimental validation methods to confirm transient interactions.
  • Consideration of cell lysis protocols to maximize experimental success.

Main Results:

  • A comprehensive overview of diverse techniques for PPI study.
  • Discussion of the strengths and limitations of each method.
  • A workflow chart to aid in experimental design and method selection.

Conclusions:

  • Appropriate selection of techniques is critical for successful PPI studies.
  • Knowledge of PPIs enhances understanding of cellular networks and disease pathways.
  • This review serves as a reference for researchers navigating PPI methodologies.