Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

5.6K
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
5.6K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

13.6K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
13.6K
Protein Transport into the Inner Mitochondrial Membrane01:34

Protein Transport into the Inner Mitochondrial Membrane

5.1K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
5.1K
Peroxisomes01:24

Peroxisomes

22.0K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
22.0K
Peroxisomes01:24

Peroxisomes

1.8K
1.8K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

5.9K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

YdbL directly modulates YdbH-YnbE bridge formation to maintain <i>Escherichia coli</i> outer membrane homeostasis.

mBio·2026
Same author

SpsB is a target of SOS-response inhibitor OXF-077 and regulates quinolone resistance emergence.

Cell chemical biology·2026
Same author

Modular Rh-Catalyzed Synthesis and Biological Profiling of Diverse Pentafluorobenzenesulfonamide Reactive Fragments.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Discovery of SHANK1-PDZ Peptide-Fragment Inhibitors Using a Dynamic Ligation Screening Strategy.

Biochemistry·2026
Same author

Rational discovery of therapeutic PAK1 allosteric activators.

Cell·2026
Same author

YdbL directly modulates YdbH-YnbE bridge formation to maintain <i>Escherichia coli</i> outer membrane homeostasis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 16, 2026

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
09:01

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Published on: January 7, 2022

3.1K

Correction: Peroxisome protein import: a complex journey

Alison Baker, Thomas Lanyon-Hogg, Stuart L Warriner

    Biochemical Society Transactions
    |August 17, 2016
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Monitoring Stub1-Mediated Pexophagy
    08:26

    Monitoring Stub1-Mediated Pexophagy

    Published on: May 12, 2023

    2.1K
    Studying Protein Import into Chloroplasts Using Protoplasts
    06:29

    Studying Protein Import into Chloroplasts Using Protoplasts

    Published on: December 10, 2018

    10.4K

    Related Experiment Videos

    Last Updated: Mar 16, 2026

    Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
    09:01

    Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

    Published on: January 7, 2022

    3.1K
    Monitoring Stub1-Mediated Pexophagy
    08:26

    Monitoring Stub1-Mediated Pexophagy

    Published on: May 12, 2023

    2.1K
    Studying Protein Import into Chloroplasts Using Protoplasts
    06:29

    Studying Protein Import into Chloroplasts Using Protoplasts

    Published on: December 10, 2018

    10.4K