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Interlinked Macroporous 3D Scaffolds from Microgel Rods
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Three-dimensional macroporous graphene scaffolds for tissue engineering.
Gaurav Lalwani1, Michael D'agati1, Anu Gopalan1
1Department of Biomedical Engineering, Stony Brook University, Stony Brook, New York, 11794-5281.
Journal of Biomedical Materials Research. Part A
|August 17, 2016
Summary
Chemically cross-linked graphene oxide nanoribbon scaffolds show excellent cytocompatibility for tissue engineering. These 3D porous graphene materials support cell attachment, proliferation, and metabolic activity, paving the way for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Tissue Engineering
Background:
- Three-dimensional (3D) porous scaffolds from carbon nanomaterials are essential for tissue engineering.
- Graphene's unique properties make it promising, but scaffold fabrication and biocompatibility require investigation.
Purpose of the Study:
- To fabricate and characterize chemically cross-linked 3D graphene scaffolds from single- and multiwalled graphene oxide nanoribbons (SWGONRs and MWGONRs).
- To evaluate the in vitro cytocompatibility of these graphene scaffolds using human adipose-derived stem cells (ADSCs) and murine MC3T3 preosteoblast cells.
Main Methods:
- Radical-initiated thermal cross-linking of SWGONRs and MWGONRs to create macroscopic 3D scaffolds.
- Characterization of scaffold porosity and pore interconnectivity.
- In vitro cell viability, metabolic activity, spreading, attachment, and proliferation assays using ADSCs and MC3T3 cells.
Main Results:
- Fabricated true 3D graphene scaffolds with tunable porosity (65-80%) and interconnected macro-, micro-, and nanoscale pores.
- Demonstrated good cell viability and metabolic activity of ADSCs and MC3T3 cells on SWGONR and MWGONR scaffolds over 5 days, comparable to PLGA scaffolds.
- Confirmed cell attachment and proliferation via immunofluorescence imaging of vinculin and Ki-67.
Conclusions:
- Chemically cross-linked SWGONR and MWGONR scaffolds are cytocompatible for cell growth and proliferation.
- These 3D graphene scaffolds offer a promising platform for developing multifunctional materials in tissue engineering and regenerative medicine.
