Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.6K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

2.4K
2.4K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

2.1K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
2.1K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.7K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.7K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

2.0K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analytical implications of zero-order operation and blazed gratings in chromatographic fluorescence detectors.

Analytica chimica acta·2026
Same author

Hexahydroquinoline Featuring Amide Functionality: A Promising Scaffold With Calcium Channel Blocking Activity.

Drug development research·2026
Same author

A Distinct Anion Separation Approach via Isocratic Liquid Chromatography Coupled with Vacuum Ultraviolet Detection.

Analytical chemistry·2026
Same author

Mirror-Image β-l-Cyclodextrin as a Chiral Pseudophase in Capillary Electrophoresis.

Electrophoresis·2025
Same author

Kinetic investigation on d-amino acid containing peptides and carboxypeptidase Y.

Archives of biochemistry and biophysics·2025
Same author

Back to basic: Using ammonium hydroxide to improve peptide epimer/isomer liquid chromatography separations.

Analytica chimica acta·2025

Related Experiment Video

Updated: Mar 16, 2026

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.8K

Salient Sub-Second Separations.

M Farooq Wahab1, Rasangi M Wimalasinghe1, Yadi Wang1

  • 1Department of Chemistry and Biochemistry, University of Texas at Arlington , Arlington, Texas 76019, United States.

Analytical Chemistry
|August 17, 2016
PubMed
Summary

Sub-second liquid chromatography using short columns achieves rapid separation of biologically important molecules. This technique enhances screening and two-dimensional liquid chromatography applications.

More Related Videos

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
09:48

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

27.2K
Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
08:40

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Published on: February 14, 2019

8.8K

Related Experiment Videos

Last Updated: Mar 16, 2026

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

11.8K
Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
09:48

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Published on: October 9, 2013

27.2K
Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
08:40

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting

Published on: February 14, 2019

8.8K

Area of Science:

  • Analytical Chemistry
  • Chromatography Science

Background:

  • Traditional liquid chromatography can be time-consuming.
  • Optimizing separation speed is crucial for high-throughput analysis.

Purpose of the Study:

  • To demonstrate sub-second liquid chromatography (LC) in very short packed beds.
  • To explore its potential for chiral, achiral, and Hydrophilic Interaction Liquid Chromatography (HILIC) separations.

Main Methods:

  • Utilized 0.5-cm-long columns packed with superficially porous particles (SPP) of various surface chemistries (teicoplanin, cyclofructan, silica, quinine).
  • Employed modified Ultra-High-Performance Liquid Chromatography (UHPLC) hardware, minimizing extra-column dispersion and using high data acquisition rates.
  • Applied power transform for data analysis.

Main Results:

  • Achieved sub-second separations for diverse biologically relevant molecules.
  • Demonstrated reduced plate heights between 2.6 and 3.0.
  • Confirmed the utility of power transform for enhancing sub-second chromatography.

Conclusions:

  • Sub-second LC in short packed beds is a viable proof of concept for various separation types.
  • Hardware modifications and data processing are key to maximizing performance.
  • This approach offers significant benefits for fast screening and 2D-LC.