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Integral membrane proteins in proteomics. How to break open the black box?
Journal of Proteomics
|August 18, 2016
Summary
Integral membrane proteins (IMPs) are crucial for cell function but difficult to study. Recent advances in membrane proteomics offer new strategies for analyzing these vital proteins.
Area of Science:
- Proteomics
- Molecular Biology
- Biochemistry
Background:
- Integral membrane proteins (IMPs) perform essential cellular functions.
- IMPs are encoded by 20-30% of human genes.
- Their hydrophobicity and low expression limit proteomic analysis.
Purpose of the Study:
- To review recent developments in membrane proteomics.
- To outline successful strategies for integral membrane protein analysis.
Main Methods:
- Filter-assisted sample preparation (FASP).
- Cell surface capture (CSC) protocols.
- Methods for analyzing hydrophobic transmembrane segments.
Main Results:
- Significant advancements have been made in membrane proteomics.
- New techniques enable better analysis of IMPs.
- Successful strategies for large-scale IMP analysis have been identified.
Conclusions:
- Despite challenges, membrane proteomics is rapidly advancing.
- New methods improve the study of integral membrane proteins.
- This review provides a guide to current successful strategies.
