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Monitoring Protein Adsorption with Solid-state Nanopores
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Probing protein adsorption on a nanoparticle surface using second harmonic light scattering.

A Das1, A Chakrabarti, P K Das

  • 1Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore 560012, India. pkdas@ipc.iisc.ernet.in.

Physical Chemistry Chemical Physics : PCCP
|August 18, 2016
PubMed
Summary

Second harmonic light scattering quantifies protein adsorption onto gold nanoparticles. This method reveals binding thermodynamics and is reversible, crucial for nanoparticle diagnostics and therapeutics.

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Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Protein-nanoparticle interactions are vital for diagnostics and therapeutics.
  • Quantifying these interactions, especially weak physisorption, remains challenging.
  • Gold nanoparticles offer unique optical properties for surface probing.

Purpose of the Study:

  • To introduce and validate second harmonic light scattering (SHLS) for studying protein physisorption on gold nanoparticles.
  • To determine thermodynamic parameters like free energy of adsorption and binding affinity for proteins.
  • To investigate the influence of gold nanoparticle size on protein adsorption.

Main Methods:

  • Utilized SHLS to monitor changes in scattered light intensity as a function of protein binding.
  • Employed alcohol dehydrogenase (ADH) and insulin as model proteins.
  • Tested gold nanoparticles ranging from 15 to 60 nm in diameter.

Main Results:

  • SHLS successfully quantified protein adsorption and determined binding thermodynamics.
  • Adsorption free energy, binding affinity, and molecule count increased with nanoparticle size.
  • Protein desorption was achieved quantitatively via centrifugation, demonstrating reversibility.

Conclusions:

  • SHLS is a powerful, reversible method for probing biomolecule-nanoparticle interactions.
  • Findings are critical for developing nanoparticle-based diagnostic and therapeutic formulations.
  • The study highlights the importance of nanoparticle size in modulating adsorption thermodynamics.