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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

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Related Experiment Video

Updated: Mar 16, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Splicing-factor alterations in cancers.

Olga Anczuków¹, Adrian R Krainer¹

¹Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.

RNA (New York, N.Y.)

|August 18, 2016

Summary

This summary is machine-generated.

Alterations in RNA splicing factors are common in human cancers, affecting gene expression. This review explores these splicing factor changes, their mechanisms, and potential therapeutic strategies targeting cancer splicing events.

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Last Updated: Mar 16, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Published on: April 22, 2021

Area of Science:

Molecular Biology
Oncology
Genetics

Background:

Tumorigenesis involves alterations in RNA splicing.
Splicing factor changes can arise from mutations or machinery disruptions.

Purpose of the Study:

To review the role of splicing-factor alterations in human cancers.
To discuss mechanisms of splicing regulation in normal and cancerous cells.
To explore novel therapeutic strategies targeting cancer splicing.

Main Methods:

Literature review of splicing-factor alterations in human tumors.
Analysis of splicing changes and their cell-type specificities.
Examination of splicing factor regulation mechanisms.

Main Results:

Splicing-factor alterations are detected across various human tumors.
These alterations lead to significant changes in RNA splicing patterns.
Cell-type specificities in splicing alterations and outcomes are highlighted.

Conclusions:

Splicing factor alterations are a key feature of human cancers.
Understanding these alterations provides insights into cancer development.
Targeting oncogenic splicing events offers promising therapeutic avenues.