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Mouse serum contains two erythroid progenitor-stimulating activities, one being highly increased in serum from anemic
S Arnaud1, G Mouchiroud, M Arnaud
1Laboratory of Cell Biology, Claude Bernard University, Villeurbanne, France.
Abstract:
We previously observed that the addition of normal mouse serum to mouse bone marrow cell cultures increases the number of erythroid colonies arising from erythroid colony-forming units (CFU-E). In this paper, we show that the stimulating activity could be divided into two active fractions with 110 kd and 50 kd apparent molecular weight by gel filtration. The 50-kd fraction, in addition to its stimulating activity, increased the sensitivity of CFU-E to erythropoietin. The 110-kd activity was hardly detectable in normal mouse serum but was greatly increased in anemic mouse serum. This renders this activity of interest and suggests that it may play a regulatory role in mouse erythropoiesis.