Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.4K
CRISPR01:59

CRISPR

58.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.7K
CRISPR01:59

CRISPR

18.8K
18.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.5K
Homologous Recombination02:31

Homologous Recombination

65.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering plant architecture in the ornamental species Eustoma grandiflorum by knockout of strigolactone biosynthesis.

Plant cell reports·2026
Same author

CRISPR/Cas9-mediated genome editing reveals the involvement of a polyphenol oxidase in the shikonin-specific biosynthesis in Lithospermum erythrorhizon.

Plant & cell physiology·2026
Same author

Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteases.

Journal of virology·2026
Same author

Haplotype bias detection using pedigree-based transmission simulation: traces of selection that occurred in apple breeding.

Horticulture research·2026
Same author

Skeletal muscle-specific PGC-1α-b overexpression prevents eccentric contraction-induced muscle injury through an utrophin-independent pathway in mice.

Physiological reports·2026
Same author

Guidelines for Nutritional Management of Sarcopenia and Frailty 2025. Guest Editor: Masafumi Kuzuya. This publication has been supported by The Japan Geriatrics Society and National Center for Geriatrics and Gerontology (NCGG) (Japan).

Geriatrics & gerontology international·2026

Related Experiment Video

Updated: Mar 16, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.2K

Efficient Genome Editing in Apple Using a CRISPR/Cas9 system.

Chikako Nishitani1, Narumi Hirai1, Sadao Komori2

  • 1NARO Institute of Fruit Tree and Tea Science, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605, Japan.

Scientific Reports
|August 18, 2016
PubMed
Summary

Genome editing successfully created targeted mutations in apple genes using CRISPR/Cas9 technology. This breakthrough enables practical genome modification for developing novel fruit tree traits.

More Related Videos

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K

Related Experiment Videos

Last Updated: Mar 16, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

36.2K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K

Area of Science:

  • Plant biotechnology
  • Molecular genetics
  • Agricultural science

Background:

  • Genome editing offers significant advantages for modifying plant genetics, but its application in fruit trees is still developing.
  • The CRISPR/Cas9 system is a key tool for precise genome modification.

Purpose of the Study:

  • To establish genome editing in apple plantlets using the CRISPR/Cas9 system.
  • To induce targeted mutations in the apple phytoene desaturase (PDS) gene.

Main Methods:

  • Designing and transforming four guide RNAs (gRNAs) with Cas9 in apple.
  • Regenerating plantlets and analyzing phenotypes for induced mutations.
  • Confirming bi-allelic mutations in the apple PDS gene via DNA sequencing.

Main Results:

  • Albino phenotypes were observed in 31.8% of regenerated plantlets using one specific gRNA.
  • Targeted mutations were confirmed in the apple PDS gene.
  • Evidence suggests both NHEJ and HR pathways were involved in the CRISPR/Cas9-induced mutations.

Conclusions:

  • CRISPR/Cas9-mediated genome editing is a viable technique for modifying the apple genome.
  • This study demonstrates the practical application of genome editing in fruit tree improvement.
  • The findings pave the way for introducing desirable traits in apple varieties.