Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 16, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

18.2K

The time-resolved transcriptome of C. elegans.

Max E Boeck1, Chau Huynh2, Lou Gevirtzman2

  • 1Department of Genome Sciences, School of Medicine, University of Washington, Seattle, Washington 98195, USA; Department of Biology, Regis University, Denver, Colorado 80221, USA.

Genome Research
|August 18, 2016
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medicaid Asset Limits And Enrollment Among Older Adults And People With Disabilities.

Health affairs (Project Hope)·2026
Same author

Unravelling ring chromosome structures and formation mechanisms by short-read and long-read genomic sequencing.

Genetics in medicine open·2026
Same author

Β-Resorcylaldehyde attenuates DSS-induced colitis by inhibiting inflammation and oxidative stress through the Keap1/Nrf2/HO-1 pathway.

International immunopharmacology·2026
Same author

Perioperative hyperchloremia is associated with acute kidney injury in elderly patients undergoing bipolar plasmakinetic transurethral resection of the prostate: a prospective observational study.

World journal of urology·2026
Same author

Rethinking intraoperative blood loss monitoring: a decision-oriented framework for clinically integrated assessment.

International journal of burns and trauma·2026
Same author

Multi-scale dissection, compaction and derivatization of mammalian developmental enhancers.

bioRxiv : the preprint server for biology·2026

This study provides high-resolution RNA-seq data for Caenorhabditis elegans, revealing dynamic gene expression patterns during development and uncovering novel splice junctions and transcriptional programs. The findings enhance our understanding of the nematode transcriptome.

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Understanding gene expression dynamics is crucial for deciphering developmental processes.
  • The nematode Caenorhabditis elegans serves as a powerful model organism for studying gene regulation.
  • Comprehensive transcriptome annotation is essential for accurate biological interpretation.

Purpose of the Study:

  • To generate high-resolution RNA-seq data for Caenorhabditis elegans across its life cycle.
  • To identify dynamic changes in gene expression during embryogenesis and post-embryonic development.
  • To discover novel transcriptional features, including alternative splicing and promoter usage.

Main Methods:

  • RNA sequencing (RNA-seq) with high temporal resolution.

More Related Videos

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

11.0K
Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

4.2K

Related Experiment Videos

Last Updated: Mar 16, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

18.2K
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
10:37

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Published on: March 17, 2019

11.0K
Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

4.2K
  • Analysis of gene expression profiles across embryonic and post-embryonic stages.
  • Identification and annotation of splice junctions, promoter usage, and transcriptional programs.

    • Main Results:

    • Gene expression changes significantly during early and late embryogenesis, with a lull in mid-embryogenesis.
    • Histone mRNAs constitute a substantial portion of RNA during mid-embryogenesis.
    • Numerous unannotated splice junctions and alternative splice forms were identified, with differential usage across the life cycle.
    • Internal promoter usage in operons was annotated using SL1 and SL2 data.
    • Correlated transcriptional programs spanning over 80 kb were uncovered.

    Conclusions:

    • The generated RNA-seq data provide a detailed annotation of the C. elegans transcriptome.
    • Developmental stages exhibit distinct patterns of gene expression regulation.
    • The study reveals extensive alternative splicing and novel regulatory elements in the C. elegans genome.