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[Differentiation of K562 Cells Induced by Pulsatilla Saponin A into Erythroid Lineage]
Tong Wang1, Ri Zhang1, Xiao-Fei Qi2
1Collaborative Innovation Center of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Jiangsu Institute of Hematology, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province,China.
Objective:
To explore the differentiation-inducing potentiality of Pulsatilla saponin A on K562 cells.
Methods:
Pulsatilla saponin A of different concentrations was used to treat K562 cells; the benzidine staining and the hemoglobinometry were applied to measure the change of hemoglobin content; the flow cytometry (FCM) was used to detect the expression of CD71 and GPA on K562 cells.
Results:
K562 cells treated with 4 µg/ml pulsatilla saponin A differentiated into the erythroid lineage. With the treatment of pulsatilla saponin A, the hemoglobin content in K562 cells increased significantly; CD71 and GPA expression on the K562 cell surface were up-regulated.
Conclusion:
Pulsatilla saponin A can induce K562 cells to differentiate into erythroid lineage.
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