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Natural Functional SNPs in miR-155 Alter Its Expression Level, Blood Cell Counts, and Immune Responses

Congcong Li1, Huabin He2, An Liu3

  • 1Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China; College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, China.

Frontiers in Immunology
|August 18, 2016
PubMed

Insights

Single nucleotide polymorphisms (SNPs) in microRNA-155 (miR-155) influence immune responses in mice and humans. These miR-155 genetic variations impact blood parameters and inflammatory responses, offering insights into immune-related diseases.

Area of Science:

  • Genetics
  • Immunology
  • Molecular Biology

Background:

  • MicroRNA-155 (miR-155) is a critical regulator of immune responses in mammals.
  • Understanding genetic variations in miR-155 can elucidate individual differences in immunity.
  • Single nucleotide polymorphisms (SNPs) are common genetic variations that can alter gene function.

Purpose of the Study:

  • To identify and characterize functional SNPs in miR-155 in mice and humans.
  • To investigate the impact of these SNPs on miR-155 expression and immune parameters.
  • To explore the potential of these SNPs as markers for immune-related diseases.

Main Methods:

  • Identification of four SNPs in miR-155 in mice and humans.
  • Genotyping of mice and analysis of blood parameters under normal and lipopolysaccharide (LPS) stimulated conditions.
  • Assessment of inflammatory response, survival rates after infection, and secondary RNA structure predictions using RNAfold.
  • Analysis of SNPs in pre-miR-155 and miR-155* regions.

Main Results:

  • Four linked SNPs in mice formed two haplotypes, associated with differential blood parameters and immune responses.
  • Mice with the AA genotype exhibited increased miR-155 expression, heightened inflammatory response to LPS, and higher mortality after Salmonella typhimurium infection.
  • Two SNPs in mice and two in humans were identified as responsible for differential miR-155 expression and function by affecting secondary RNA structure.
  • Human miR-155 SNPs may serve as causal mutations for immune-related diseases.

Conclusions:

  • Natural functional SNPs in miR-155 exist in both mice and humans, modulating mature miR-155 expression via secondary structure changes.
  • These findings highlight the role of miR-155 genetic variations in immune system regulation.
  • Mouse models with distinct miR-155 genotypes can be valuable for studying immune diseases linked to aberrant miR-155 expression.
Keywords:
blood parametersexpression levelimmune responsemiR-155natural functional SNP

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