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Do cadmium and dexamethasone induce different types of metallothioneins?
R A Andersen1, H L Daae, A Mikalsen
1Department of Toxicology, National Institute of Public Health, Oslo, Norway.
General Pharmacology
|January 1, 1989
Abstract:
1. Dexamethasone induced thionein in rat liver cross reacts with antibody made towards Cd-thionein from rat liver. 2. Dex-thionein binds Cd. 3. Compared to Cd-thionein, dex-thionein behaves differently on SDS polyacrylamide gels. 4. On molecular filtration on Sephadex G-75 columns, dex-thionein elutes slightly more towards the low molecular weight region than Cd-thionein. 5. Dex-thionein elutes in one peak with two shoulders after DEAE-Sephadex A-50 ion exchange chromatography, while Cd-thionein elutes in two peaks.