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Cloning of the amiA locus of Streptococcus pneumoniae and identification of its functional limits
G Alloing1, M C Trombe, J P Claverys
1Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Université Paul Sabatier, Toulouse, France.
Gene
|January 1, 1989
Abstract:
Mutations in the amiA locus of the Gram-positive bacterium Streptococcus pneumoniae confer a complex phenotype including resistance to various antineoplastic drugs. As a first step towards the understanding of the molecular organization and the function(s) of this locus, we have cloned DNA fragments carrying its 5'- and 3'-extremities. We have isolated and characterized a down-promoter mutation and have located the functional limits of the locus. The amiA locus is between 5.8 and 7.5 kb long strongly suggesting that it encodes several proteins.