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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

10.4K

Human INO80/YY1 chromatin remodeling complex transcriptionally regulates the BRCA2- and CDKN1A-interacting protein

Jiaming Su1, Yi Sui1, Jian Ding1

  • 1School of Life Sciences, Jilin University, 2699 Qianjin Street, Changchun, 130012, China.

Protein & Cell
|August 19, 2016
PubMed

Insights

BRCA2- and CDKN1A-interacting protein (BCCIP) is crucial for tumor suppression. This study reveals BCCIP is a novel target gene of the INO80/YY1 chromatin remodeling complex, clarifying its cellular regulation mechanism.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Chromatin Biology

Background:

  • BCA2- and CDKN1A-interacting protein (BCCIP) is a tumor suppressor cofactor with low expression in various cancers.
  • The precise regulatory mechanisms of BCCIP expression in cells remain largely unknown.
  • The INO80/YY1 complex is a human chromatin remodeling complex involved in nucleosome dynamics.

Purpose of the Study:

  • To investigate and identify novel regulators of BCCIP expression.
  • To determine if the INO80/YY1 complex targets BCCIP.
  • To elucidate the mechanism by which INO80/YY1 influences BCCIP gene expression.

Main Methods:

  • Gene expression analysis and siRNA knockdown experiments to identify candidate genes.
  • Luciferase reporter assays to assess transcriptional regulation.
  • Chromatin immunoprecipitation (ChIP) assays to determine protein-DNA interactions.

Main Results:

  • Gene expression studies and siRNA knockdown identified BCCIP as a target of the INO80/YY1 complex.
  • Silencing or overexpressing INO80/YY1 subunits altered BCCIP mRNA and protein levels.
  • ChIP assays confirmed enrichment of INO80 and YY1 at the BCCIP promoter, dependent on both proteins.

Conclusions:

  • BCCIP is a novel target gene of the human INO80/YY1 chromatin remodeling complex.
  • The INO80/YY1 complex plays a critical role in regulating BCCIP gene expression.
  • These findings provide new insights into the regulation of BCCIP and its role in tumor suppression.
Keywords:
BCCIPchromatin remodeling complexhuman INO80

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