Gene editing for cell engineering: trends and applications.

Area of Science:

• Molecular Biology
• Biotechnology
• Genetic Engineering

Background:

• Gene editing tools like CRISPR, TALENs, and ZFNs facilitate host genome manipulation.
• Industrial production hosts such as yeast and mammalian cells can be modified for cost-effective recombinant protein development.

Purpose of the Study:

• To review modern gene editing tools for host genome modification.
• To emphasize CRISPR-Cas9's multiplexing capabilities for yeast and CHO cell genetic manipulation.

Main Methods:

• Review of CRISPR, TALENs, and ZFNs gene editing technologies.
• Focus on multiplexed gene editing strategies for knock-in/knock-out events.

Main Results:

• Multiplexed gene editing revolutionizes knock-out and knock-in events in yeast and CHO cells.
• Enables high, stable, and consistent transgene expression for therapeutic proteins like monoclonal antibodies.

Conclusions:

• CRISPR-Cas9 facilitates rapid genetic manipulation of production hosts.
• Leads to faster product development with improved yield, quality, and affordability.