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Mass spectrometry based data of the blister fluid proteome of paediatric burn patients

Tuo Zang1, Daniel A Broszczak1, Leila Cuttle2

  • 1Tissue Repair and Regeneration Program, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia; School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia; Wound Management Innovation Co-operative Research Centre, Brisbane, Queensland, Australia.

Data in Brief
|August 19, 2016
PubMed

Insights

This study analyzed proteins in paediatric burn blister fluid, identifying key proteins involved in wound healing and stress response. These findings offer insights into the biological processes underlying burn injuries in children.

Area of Science:

  • Biochemistry
  • Proteomics
  • Paediatric Medicine

Background:

  • Burn injuries are traumatic for children, with severity influencing scar formation and requiring extensive treatment.
  • Understanding the molecular mechanisms of burn healing is crucial for improving patient outcomes.

Purpose of the Study:

  • To characterize the proteome of paediatric burn blister fluid.
  • To identify proteins associated with different burn depths and biological processes like wound healing and stress response.

Main Methods:

  • Paediatric burn blister fluid samples were fractionated and pooled based on burn depth.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for proteomic analysis.
  • Gene Ontology enrichment analysis was performed to identify over-represented biological functions and pathways.

Main Results:

  • A comprehensive table of identified proteins, their associated burn depths, sequence coverage, and peptide identifications was generated.
  • Significant over-representation of biological processes, molecular functions, and cellular components was observed in the burn blister fluid proteome.
  • Proteins linked to "wound healing" and "response to stress" were identified, highlighting their relevance in burn wound pathophysiology.

Conclusions:

  • The study provides a detailed proteomic profile of paediatric burn blister fluid.
  • The identified proteins and pathways offer valuable insights into the complex biological responses to paediatric burn injuries.
  • This data can inform future research on therapeutic targets for improved burn wound management in children.

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