Related Experiment Video

Updated: Mar 16, 2026

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation
09:53

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

Published on: January 10, 2025

908

Atorvastatin Protects Myocardium Against Ischemia-Reperfusion Injury Through Inhibiting miR-199a-5p

YaBei Zuo1, YuZhao Wang, HaiJuan Hu

  • 1Department of Cardiology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|August 19, 2016
PubMed
Abstract

Insights

Atorvastatin protects heart cells from injury by increasing GSK-3β expression and inhibiting miR-199a-5p. This mechanism enhances cell viability after ischemia/reperfusion (I/R) events.

Area of Science:

  • Cardiovascular Research
  • Molecular Cardiology
  • Pharmacology

Background:

  • Myocardial ischemia/reperfusion (I/R) injury is a significant cause of heart damage.
  • Understanding protective mechanisms against I/R injury is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the cardioprotective effects of atorvastatin against I/R injury in cardiomyocytes.
  • To elucidate the underlying molecular mechanism involving GSK-3β and miR-199a-5p.

Main Methods:

  • Cardiomyocytes were subjected to oxygen-glucose deprivation/reperfusion (OGD/R) with or without atorvastatin pretreatment.
  • Gene and protein expression of GSK-3β and miR-199a-5p were analyzed using RT-PCR, Western blot, and immunohistochemistry.

Main Results:

  • Atorvastatin pretreatment significantly improved cardiomyocyte viability following OGD/R.
  • Atorvastatin increased GSK-3β expression (mRNA and protein) and decreased miR-199a-5p expression (mRNA).
  • Modulating miR-199a-5p levels directly affected GSK-3β expression, confirming a regulatory relationship.

Conclusions:

  • Atorvastatin exerts cardioprotective effects against I/R injury.
  • The mechanism involves atorvastatin-induced upregulation of GSK-3β via inhibition of miR-199a-5p.

More Related Videos

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury07:23

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Published on: March 7, 2022

7.5K
Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy07:40

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy

Published on: May 26, 2023

1.7K

Related Concept Videos

Lipid-Lowering Drugs: Statins and Miscellaneous Agents01:20

Lipid-Lowering Drugs: Statins and Miscellaneous Agents

Hyperlipidemia, a medical condition often referred to as high cholesterol, is characterized by abnormally elevated levels of lipids in the bloodstream. When present in excess, these lipids, specifically cholesterol and triglycerides, can lead to serious health complications, often involving cardiovascular diseases. Illnesses like atherosclerosis, heart attacks, and pancreatitis have all been linked to untreated hyperlipidemia. This means controlling and regulating cholesterol and triglyceride...
1.7K
Ischemic Heart Disease: Overview01:17

Ischemic Heart Disease: Overview

Ischemic heart disease occurs when the heart's blood supply dwindles, causing an ominous lack of oxygen and nutrients. This deficiency, stemming from reduced or obstructed blood flow, spells danger, leading to heart muscle damage and dysfunction.
Atherosclerosis, the primary malefactor, orchestrates this dangerous condition. It manifests as the accumulation of fatty deposits, akin to insidious plaques, within arterial walls. As time elapses, these plaques metamorphose, hardening and...
3.8K
Myocarditis III: Medical Management01:14

Myocarditis III: Medical Management

Myocarditis: Comprehensive Medical ManagementMyocarditis, the heart muscle inflammation, requires a comprehensive medical management strategy that addresses the underlying cause, provides supportive care, manages symptoms, and reduces cardiac workload.Infections and Autoimmune CausesAdminister appropriate antimicrobial therapy when an infectious agent causes myocarditis. For instance, penicillin treats infections caused by Group A Streptococcus. In cases where autoimmune processes are...
299
Coronary Artery Disease V: Interprofessional Care01:27

Coronary Artery Disease V: Interprofessional Care

Interprofessional care for coronary artery disease includes pharmacological therapy and revascularization procedures.Pharmacological therapy for Coronary Artery Disease (CAD) aims to manage symptoms, prevent complications, and improve patient outcomes through various classes of medications:Antiplatelet Agents:Aspirin and Clopidogrel: These medications inhibit platelet aggregation, preventing blood clots, which is crucial for avoiding heart attacks and strokes. Doctors often prescribe these...
363