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Related Experiment Video

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Construction and Characterization of a Novel Vocal Fold Bioreactor
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A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications.

Neda Latifi1, Hossein K Heris1, Scott L Thomson2

  • 11 Department of Mechanical Engineering, McGill University , Montreal, Canada .

Tissue Engineering. Part C, Methods
|August 19, 2016
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Summary

Researchers developed a novel bioreactor that mimics human vocal fold (VF) phonation. This device supports cell viability and extracellular matrix synthesis, paving the way for testing VF biomaterials and understanding voice disorders.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Voice Research

Background:

  • Human vocal folds (VFs) experience complex biomechanical forces during speech.
  • Understanding these forces is crucial for developing effective treatments for voice disorders and improving biomaterials.

Purpose of the Study:

  • To develop and validate a phono-mimetic VF flow perfusion bioreactor.
  • To mimic the mechanical microenvironment of human VFs in vitro.
  • To assess cell viability and extracellular matrix synthesis within the bioreactor.

Main Methods:

  • Constructed a bioreactor with synthetic VF replicas and a cell-scaffold mixture (CSM) of VF fibroblasts, hyaluronic acid, gelatin, and PEG.
  • Used airflow-induced self-oscillations to simulate phonation, with a non-stimulated control group.
  • Perfused cell culture medium (CCM) and harvested CSM after 7 days for analysis.

Main Results:

  • The phono-mimetic bioreactor successfully supported cell viability and extracellular matrix protein synthesis.
  • Some scaffold materials degraded due to phonation-induced biomechanical stress and biochemical reactions.
  • The bioreactor provided a platform to study VF cell behavior under simulated phonation.

Conclusions:

  • The developed bioreactor effectively replicates the mechanical conditions of human vocal fold phonation in vitro.
  • It serves as a valuable tool for investigating the effects of different voice regimes on VF cells.
  • This system facilitates the preclinical evaluation of VF-specific biomaterials for future clinical applications.