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Cloning and characterization of bovine cytochrome P-450(11 beta) genes
T Hashimoto1, K Morohashi, T Omura
1Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka.
Journal of Biochemistry
|May 1, 1989
Abstract:
We isolated 4 different clones of the P-450(11 beta) gene from a bovine genomic library. These genomic clones were highly homologous with each other. Two of the isolated clones were pseudogenes. Determination of its nucleotide sequences indicated that the bovine P-450(11 beta) gene is divided into 9 exons by 8 introns and that it is about 8.5 kb in total length. The number of exons and the locations of intron insertion into the P-450(11 beta) gene are identical with those in the case of P-450(SCC), but different from those of other microsomal P-450s.