Related Experiment Video
Updated: Jul 20, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Example study for granular bioreactor stratification: Three-dimensional evaluation of a sulfate-reducing granular
Tian-Wei Hao1, Jing-Hai Luo1, Kui-Zu Su2
1Department of Civil &Environmental Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
Biomass stratification in granular sludge bioreactors is key for treating sulfate-laden wastewater. The reactor substratum, rich in diverse sulfate-reducing bacteria (SRB) and acidogens, acts as the main acidification zone.
Area of Science:
- Environmental Microbiology
- Biochemical Engineering
Background:
- Sulfate-reducing granular sludge is emerging for sulfate-laden water and wastewater treatment.
- Biomass stratification within granular bioreactors remains poorly understood, impacting bioprocess efficiency.
Purpose of the Study:
- To investigate biomass stratification, hydrodynamics, and microbial communities in a granular sludge bioreactor.
- To elucidate the role of different sludge layers in sulfate removal and acidification.
Main Methods:
- Comprehensive analysis of sludge layer performance, internal hydrodynamics, and microbial community structure.
- Computational fluid dynamics (CFD) simulations for visualizing hydrodynamics.
- 16S rRNA gene sequencing for microbial community analysis.
Main Results:
- The reactor substratum (below baffle 1) was identified as the primary acidification zone.
- Baffles enhanced mixing but created dead zones.
- More diverse sulfate-reducing bacteria (SRB) and acidogen communities were found in the substratum compared to the superstratum.
Conclusions:
- Biomass stratification significantly influences bioreactor performance.
- Understanding sludge layer characteristics aids in designing and optimizing SRB granular bioreactors for effective wastewater treatment.
More Related Videos
Related Concept Videos
The Winogradsky Column
Microbial Mats
Bioreactor Design and Operational System
Bioreactor Controls-II

