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Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: Point-of-Care Ultrasound for Gastric Content Assessment and Risk Stratification in Perioperative Care

Author Spotlight: Point-of-Care Ultrasound for Gastric Content Assessment and Risk Stratification in Perioperative Care

Published on: September 22, 2023

Gastric Aspiration Models.

Bruce A Davidson¹, Ravi Alluri¹

¹Department of Anesthesiology, and Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, USA.

|August 20, 2016

Summary

This summary is machine-generated.

This study details methods for inducing gastric aspiration pneumonitis in rodents. Researchers investigate inflammatory responses using various injury vehicles, finding volume, pH, and particle concentration impact lung injury severity.

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Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Open Tracheostomy Gastric Acid Aspiration Murine Model of Acute Lung Injury Results in Maximal Acute Nonlethal Lung Injury

Published on: February 26, 2017

Area of Science:

Pulmonary Medicine
Toxicology
Animal Models

Background:

Gastric aspiration can cause severe lung injury.
Understanding inflammatory responses is crucial for developing treatments.

Purpose of the Study:

To establish and describe reproducible methods for inducing gastric aspiration pneumonitis in mice, rats, and rabbits.
To investigate the impact of different injury vehicles on inflammatory responses and lung injury severity.

Main Methods:

Utilized four intratracheal injury vehicles: normal saline, acid (HCl pH 1.25), gastric particles (pH ~5.3), and a combination of acid and particles.
Standardized injury volumes, pH levels, and particle concentrations for mice, rats, and rabbits.
Emphasized the importance of empirical determination of particulate concentration for new strains, especially genetically altered mice.

Main Results:

Volume, pH, and gastric particle concentration significantly influence the severity of lung injury.
The acid + particle vehicle induced maximal non-lethal lung injury with ≤10% mortality in mice.
Strain-specific immune bias and mouse size (≥20g) are critical considerations for data interpretation and surgical success.

Conclusions:

The described procedures provide a robust model for studying gastric aspiration pneumonitis and associated inflammatory responses.
Careful consideration of animal model (strain, size) and injury characteristics is essential for accurate research outcomes.
These methods facilitate research into the pathogenesis and potential therapeutic interventions for aspiration-induced lung injury.