Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early-life exposure to 27.5 GHz 5G millimeter-wave radiation induces skin-related biological responses in mice.

Scientific reports·2026
Same author

Fecal Proteomics Suggest Potential Biomarkers for Non-Alcoholic Fatty Liver Disease and Steatohepatitis.

Proteomics. Clinical applications·2026
Same author

Targeted delivery of a plant-derived monoclonal antibody using pulsed electric fields in a 3D glioblastoma model.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Fecal miRNome and Proteome Profiling Uncovers Stage-Specific Biomarkers of Alzheimer's Disease in 3×Tg-AD Mice.

Cellular and molecular neurobiology·2026
Same author

AMBRA1 enhances Sonic Hedgehog signaling during cerebellar development and in medulloblastoma.

Developmental cell·2026
Same author

Deregulation of Synaptic Plasticity-Related MicroRNAs After Repetitive Transcranial Magnetic Stimulation in Alzheimer's Disease.

Molecular neurobiology·2026

Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery
05:19

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Published on: December 1, 2023

1.8K

Nonlinear Radiation-Induced Cataract Using the Radiosensitive Ptch1(+/-) Mouse Model.

Ilaria De Stefano1, Paola Giardullo1,2, Barbara Tanno3

  • 1b   Department of Radiation Physics, Guglielmo Marconi University, Rome, Italy;

Radiation Research
|August 20, 2016
PubMed
Summary

Low-dose radiation exposure in young mice heterozygous for the Patched1 (Ptch1) gene did not cause cataracts. A dose threshold exists for radiation-induced lens opacity and fibrotic changes in this animal model.

More Related Videos

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
13:56

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

10.4K
A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

19.0K

Related Experiment Videos

Last Updated: Mar 16, 2026

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery
05:19

Author Spotlight: Unraveling the Molecular Mechanisms in PCO and Fibrosis Following Cataract Surgery

Published on: December 1, 2023

1.8K
Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
13:56

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

10.4K
A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

19.0K

Area of Science:

  • Radiation biology
  • Ophthalmology
  • Genetics

Background:

  • Non-cancer health effects, such as lens opacity, are increasingly recognized following low-dose radiation exposure.
  • Mice heterozygous for the Patched1 (Ptch1) gene are a valuable model for studying radiation-induced cataracts.
  • Uncertainty exists regarding safe radiation dose levels for the lens and the presence of a cataract development threshold.

Purpose of the Study:

  • To investigate the effects of a range of low radiation doses (0.5 Gy, 1 Gy, 2 Gy) on the development of cataracts in young Ptch1(+/-) mice.
  • To evaluate molecular changes, including epithelial-mesenchymal transition (EMT), in response to radiation in the mouse lens.
  • To determine if a dose threshold exists for radiation-induced cataractogenesis and associated molecular alterations.

Main Methods:

  • Irradiation of Ptch1(+/-) mice at 2 days of age with varying doses of X rays (0.5 Gy, 1 Gy, 2 Gy).
  • Macroscopic and microscopic examination of lenses for cataract development.
  • Molecular analyses to assess epithelial-mesenchymal transition (EMT) and fibrotic alterations in lens cells.

Main Results:

  • Acute radiation exposure at doses up to 2 Gy did not induce macroscopically detectable cataracts in young Ptch1(+/-) mice.
  • Only mice exposed to 2 Gy showed microscopic lens alterations.
  • Molecular analyses indicated a dose threshold for radiation-induced EMT and fibrotic changes in the mouse lens model.

Conclusions:

  • A dose threshold appears to exist for radiation-induced cataractogenesis and associated molecular changes in the Ptch1(+/-) mouse model.
  • Further research is essential to fully understand the mechanisms of radiation-induced cataract initiation and development.
  • Identifying genetic factors influencing cataract susceptibility after irradiation requires additional experimental investigation.