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Related Concept Videos

Urine Studies II: Urine Culture and Sensitivity Test01:26

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A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
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Related Experiment Video

Updated: Mar 16, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
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False Negative Results in Clostridium difficile Testing.

Yanal M Murad1,2, Justo Perez3, Gustavo Ybazeta3

  • 1Current Address: Advanced Medical Research Institute of Canada, 41 Ramsey Lake Road, Sudbury, ON, P3E 5J1, Canada. yamurad@nosm.ca.

BMC Infectious Diseases
|August 21, 2016
PubMed
Summary
This summary is machine-generated.

False negative results in Clostridium difficile infection (CDI) testing can occur with polymerase chain reaction (PCR) methods. Careful consideration of diagnostic test sensitivity and clinical factors is crucial for accurate CDI diagnosis and patient management.

Keywords:
Clostridium difficileFalse negativeMolecular testing

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A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
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Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Diagnostic testing

Background:

  • Accurate diagnosis of Clostridium difficile infection (CDI) is critical for effective patient management and preventing healthcare-associated transmission.
  • Polymerase chain reaction (PCR) amplification of the toxin B gene is a sensitive method for detecting toxigenic C. difficile, but false negatives can still occur.
  • False negative diagnostic results can delay treatment and potentially contribute to the spread of CDI within healthcare settings.

Purpose of the Study:

  • To investigate discrepancies between PCR-based testing and stool culture for toxigenic Clostridium difficile.
  • To assess the impact of false negative PCR results on patient treatment and clinical outcomes.
  • To analyze the characteristics of C. difficile isolates from discrepant samples.

Main Methods:

  • Collected 50 discrepant samples over 15 months where PCR tested negative and stool culture tested positive for toxigenic C. difficile.
  • Characterized C. difficile isolates from discrepant samples using ribotyping.
  • Reviewed patient charts to evaluate the impact of discrepant results on treatment and clinical outcomes.

Main Results:

  • Fifty out of 2308 tested samples (2.17%) showed false negative PCR results for toxigenic C. difficile.
  • Ribotyping revealed diverse patterns among the C. difficile isolates, indicating different strains.
  • In most cases, false negative PCR results did not significantly impact patient clinical outcomes.

Conclusions:

  • The limit of detection of PCR assays may contribute to false negative results in C. difficile detection.
  • Both clinical and analytical sensitivity of diagnostic tests for C. difficile are important considerations.
  • Clinical correlation is essential when interpreting diagnostic results and should be carefully examined before ruling out CDI.