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Alendronate alters osteoblast activities.
Tormod B Krüger1, Bente B Herlofson1, Maria A Landin2
1a Department of Oral Surgery and Oral Medicine, Faculty of Dentistry , University of Oslo , Oslo , Norway.
Acta Odontologica Scandinavica
|August 23, 2016
Summary
Alendronate (ALN) affects human osteoblasts by enhancing immune factor secretion and altering proliferation and differentiation. High doses or accumulation may lead to adverse bone changes.
Area of Science:
- Bone biology and pharmacology
- Cellular and molecular mechanisms of bisphosphonates
Background:
- Bisphosphonates (BPs) accumulate in bone with long half-lives, necessitating understanding their cellular effects.
- Alendronate (ALN) is a widely used bisphosphonate for treating bone diseases.
Purpose of the Study:
- To investigate the impact of alendronate (ALN) on human primary osteoblasts.
- To assess ALN's effects on osteoblast proliferation, viability, and differentiation.
Main Methods:
- Human osteoblasts were treated with varying concentrations of ALN (5, 20, 100 μM).
- Cell proliferation was measured using 3H-thymidine incorporation and MTT assays.
- Cell viability, differentiation markers (mRNA expression), and immune factor secretion were analyzed.
Main Results:
- ALN did not affect osteoblast viability.
- Low ALN concentrations increased proliferation; high concentration (100 μM) decreased it.
- ALN reduced osteoblast differentiation markers but increased leptin, IL-8, and RANTES expression/secretion.
Conclusions:
- Alendronate enhances immune factor secretion from osteoblasts.
- Reduced proliferation and differentiation, coupled with immune factor release, suggest potential adverse local bone effects at higher doses or with accumulation.
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