Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Isolation and Connectivity: Population Structure of an Ectomycorrhizal Truffle in the Fragmented Mountain Landscape of the Madrean Sky Island Archipelago.

Molecular ecology·2026

Same author

Aeschynomene americana induces terminal bacteroid differentiation in Bradyrhizobium sp. USDA3516, a novel model for dalbergioid-rhizobium symbiosis.

BMC plant biology·2026

Same author

Characterization of a Core Fungal Community and Captivity-Induced Gut "Mycobiome" Change in Fowler's Toad (<i>Anaxyrus fowleri</i>).

Ecology and evolution·2026

Same author

Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.

ACS synthetic biology·2026

Same author

Unraveling the genomic and phylogenetic complexity of the under-studied microfungus <i>Basidiobolus</i>: Insights from 19 newly sequenced genomes.

Mycologia·2026

Same author

Cryptostroma corticale, a plant pathogen associated with a human immune respiratory syndrome displaying mixed viral infections of mycoviruses.

Virology·2026

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria.

Edward W Davis Ii^1,2, Alexandra J Weisberg¹, Javier F Tabima¹

¹Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.

|August 23, 2016

Summary

Gall-ID is a new web platform for identifying plant pathogenic bacteria using DNA sequences. It aids disease management by classifying bacteria and detecting virulence genes, focusing on gall-forming species.

Keywords:

Agrobacterium Average nucleotide identity Genomes Molecular diagnostics Multilocus sequence analysis Rhodococcus Taxonomy

More Related Videos

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Related Experiment Videos

Last Updated: Mar 16, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Area of Science:

Microbial genomics
Plant pathology
Bioinformatics

Background:

Understanding plant pathogen diversity is crucial for effective disease management.
Molecular identification methods are advancing, but DNA sequence analysis can be complex.
Gall-forming bacteria pose significant threats to various crops.

Purpose of the Study:

To develop a user-friendly web platform, Gall-ID, for accurate identification of plant pathogenic bacteria.
To facilitate the analysis of population structure and genetic diversity in plant pathogens.
To support the detection of virulence factors in bacterial pathogens.

Main Methods:

Utilized DNA sequence data including 16S rDNA, multilocus sequence analysis (MLSA), and whole genome sequences.
Developed Gall-ID as a web-based platform for bacterial taxonomic classification.
Integrated high-throughput sequencing read analysis for virulence gene homolog detection.
Provided downloadable pipelines for MLSA, average nucleotide identity (ANI) analysis, and core genome phylogeny construction.

Main Results:

Gall-ID successfully groups disease-associated bacteria to their taxonomic units.
The platform focuses on key gall-forming genera: Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus.
Gall-ID enables identification of virulence genes and supports phylogenetic analyses.
Included additional databases for broader plant pathogen identification.

Conclusions:

Gall-ID provides a valuable tool for researchers and diagnosticians in plant pathology.
The platform enhances the ability to manage plant diseases by improving pathogen identification and characterization.
Gall-ID contributes to understanding bacterial evolution and the impact of agricultural practices on pathogen populations.