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Updated: Mar 16, 2026
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
Published on: January 30, 2019
Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells
Vaibhav B Shah1, Christopher Aiken1
1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, USA.
Abstract:
This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
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