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Enterovirus 71 Virus Propagation and Purification.
Kristin L Shingler1, Lindsey J Organtini1, Susan Hafenstein2
1Microbiology and Immunology Department, Pennsylvania State University College of Medicine, Hershey, PA, USA.
Bio-Protocol
|August 23, 2016
Summary
Enterovirus 71 (EV71) causes severe disease and death. This study details the propagation and purification methods for producing infectious EV71 virions for research.
Area of Science:
- Virology
- Infectious Diseases
Background:
- Enterovirus 71 (EV71), a picornavirus, has been a global health concern since 1969.
- EV71 is a primary cause of hand, foot, and mouth disease.
- The virus can infect the central nervous system, leading to severe illness and mortality.
Purpose of the Study:
- To describe the procedure for propagating and purifying infectious enterovirus 71 (EV71) virions.
Main Methods:
- Viral propagation techniques.
- Purification methods for infectious virions.
Main Results:
- Successful generation of infectious EV71 virions.
Conclusions:
- The described methods enable the production of infectious EV71 virions.
- This is crucial for further research into EV71 pathogenesis and therapeutic development.
