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Related Experiment Video

Updated: Mar 16, 2026

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Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.

Siu-Leung Chau1, Zhi-Bing Huang2, Yan-Gang Song3

  • 1School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. dumdum1986@msn.com.

Molecules (Basel, Switzerland)
|August 23, 2016
PubMed
Summary

This study quantifies the chemical composition of Shenqi Fuzheng injection (SQI), a popular Chinese medicine. The analysis revealed that small molecules and sugars constitute the majority of the injection, aiding in understanding its efficacy and safety.

Keywords:
Chinese medicine formulaShenqi Fuzheng injectionchemical profilequantitative analysis

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Traditional Chinese Medicine

Background:

  • Quality control of Chinese medicine injections is difficult due to their complex chemical nature.
  • Shenqi Fuzheng injection (SQI) is a widely used traditional Chinese medicine injection.
  • Understanding SQI's chemical profile is crucial for ensuring its therapeutic efficacy and safety.

Purpose of the Study:

  • To conduct a comprehensive quantitative analysis of the chemical composition of Shenqi Fuzheng injection (SQI).
  • To establish a validated analytical method for determining small molecules, saccharides, and polysaccharides in SQI.
  • To provide quantitative data for better understanding the quality, efficacy, and safety of SQI.

Main Methods:

  • Simultaneous determination of 15 small molecular components using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS).
  • Quantitative analysis of saccharide composition (monosaccharides, disaccharides) using high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) before and after acid hydrolysis.
  • Examination of polysaccharide presence using gel permeation chromatography (GPC).
  • Method validation for linearity, sensitivity, precision, accuracy, and stability.

Main Results:

  • The developed analytical method was successfully applied to 13 commercial SQI samples.
  • Up to 94.69% (w/w) of the SQI product was quantitatively determined.
  • Small molecules constituted 0.18%-0.21% (w/w), while monosaccharide/sucrose content ranged from 53.49% to 58.2% (w/w).
  • Polysaccharides were also detected in the injection.

Conclusions:

  • A robust and validated analytical method was established for the quantitative analysis of SQI.
  • The study provides the first comprehensive quantitative chemical profile of SQI.
  • The detailed quantitative information supports evidence-based evaluation of SQI's therapeutic efficacy and safety.